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Antimicrobial Effect on Streptococcus mutans in Photodynamic Therapy using Different Light Source

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±èÀç¿ë ( Kim Jae-Yong ) - °­¸ª¿øÁÖ´ëÇб³ Ä¡°ú´ëÇÐ ¼Ò¾ÆÄ¡°úÇб³½Ç
¹ÚÈ£¿ø ( Park Ho-Won ) - °­¸ª¿øÁÖ´ëÇб³ Ä¡°ú´ëÇÐ ¼Ò¾ÆÄ¡°úÇб³½Ç
ÀÌÁÖÇö ( Lee Ju-Hyun ) - °­¸ª¿øÁÖ´ëÇб³ Ä¡°ú´ëÇÐ ¼Ò¾ÆÄ¡°úÇб³½Ç
¼­Çö¿ì ( Seo Hyun-Woo ) - °­¸ª¿øÁÖ´ëÇб³ Ä¡°ú´ëÇÐ ¼Ò¾ÆÄ¡°úÇб³½Ç
À̽ÿµ ( Lee Si-Young ) - °­¸ª¿øÁÖ´ëÇб³ Ä¡°ú´ëÇÐ ¹Ì»ý¹°Çб³½Ç

Abstract

º» ¿¬±¸ÀÇ ¸ñÀûÀº Ä¡¸é¼¼±Õ¸· Âø»öÁ¦ÀÎ erythrosineÀ» ±¤°¨°¢Á¦·Î »ç¿ëÇÏ¿© S. mutans ¿¡ ±¤¿ªµ¿ Ä¡·á¸¦ ½ÃÇàÇÏ¿´À» ¶§, µ¿ÀÏÇÑ ¿¡³ÊÁö·®À» Á¶»ç½Ã ±¤¿øÀÇ Á¾·ù¿¡ µû¸¥ Ç×±Õ´ÉÀÇ Â÷À̸¦ ºñ±³Çغ¸°íÀÚ ÇÔÀÌ´Ù. S. mutans ¸¦ °¢°¢ planktonic »óÅÂ¿Í biofilm »óÅ·ΠÇü¼ºÇÑ ÈÄ 40 ¥ìM·Î Èñ¼®ÇÑ erythrosineÀ» 3ºÐ°£ ó¸®ÇÑ ´ÙÀ½ ±¤¿øÀÇ Á¾·ù·Î Halogen, LED, Plasma arc¸¦ »ç¿ëÇÏ¿´°í °¢°¢ 30ÃÊ, 15ÃÊ, 9.5ÃÊÀÇ ±¤Á¶»ç¸¦ ½ÃÇàÇÏ¿´´Ù. ½ÇÇè Á¾·á ÈÄ °¢ ½ÇÇ豺ÀÇ CFU¸¦ ÃøÁ¤ÇÏ¿© °¢ Á¶°Ç¿¡ µû¸¥ ±¤¿ªµ¿ Ä¡·á È¿°ú¸¦ ºñ±³ÇÏ¿´´Ù. °¢ ½ÇÇ豺ÀÇ CFU´Â Åë°èÀûÀ¸·Î À¯ÀÇÇÑ Â÷ÀÌ°¡ ¾ø¾ú´Ù. µ¿ÀÏ ¿¡³ÊÁö·®À» Á¶»ç½Ã ±¤¿ªµ¿ Ä¡·áÀÇ È¿°ú´Â ±¤¿øÀÇ Á¾·ù¿Í °ü°è¾øÀÌ µ¿ÀÏÇÏ´Ù°í ÇÒ ¼ö ÀÖÀ¸¸ç, ÀÌ´Â ´Ù¾çÇÑ ±¤ Á¶»ç ±â±â¸¦ °¡Áø ÀÓ»óÇöÀå¿¡¼­µµ À¯¿ëÇÑ °á°ú¶ó ÇÒ ¼ö ÀÖ´Ù.

In a photodynamic therapy, the difference of antibacterial capacity was compared according to the type of source of light when the same quantity of energy is irradiated. After S. mutans is formed in planktonic state and biofilm state, erythrosine diluted to 40 ¥ìM was treated for 3 minutes, and as the type of light source, Halogen, LED, and Plasma arc were used, which were irradiated for 30 seconds, 15 seconds and 9.5 seconds, respectively. After the completion of the experiment, CFU of each experiment arm was measured to compare the photodynamic therapeutic effects according to each condition. The CFU of each experiment arm had no statistically significant difference. Under the same quantity of energy, the photodynamic therapeutic effect can be said to be the same regardless of types of light source, which is a useful result in the clinical field with various light irradiators.

Å°¿öµå

Photodynamic therapy; Erythrosine; Streptococcus mutans; Biofilm; Light source; Energy; Halogen; Light-Emitting Diode; Plasma arc

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